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Test Code LAB000 - Enter Specimen type, Source, and Test Name Nickel, Serum

Important Note

 

PRMC OutReach Serum Collection Instructions

1.   Prepare the patient for phlebotomy, following your normal protocol, using alcohol to disinfect the collection site. Do not use iodine-containing products.

2.   Draw blood in a plain (no additive, red on label) royal blue Vacutainer (Supply T184) tube

3.   Allow the specimen to clot for at least 30 mins but serum must be separated from cells within 2 hrs.

4.   Centrifuge the specimen to separate serum from cellular fraction.

5.   Attach a specimen identification label to a Mayo Metal-Free Specimen Vial (Supply T173).

6.   Remove the collection tube stopper. Carefully pour the serum into the vial, avoiding transfer of the cellular components of blood.

***Do not insert a pipette into the serum to accomplish transfer.

***Do not ream the specimen with a wooden stick to assist with serum transfer.

***Blood from a dialysis patient on heparin may produce an uncoagulated serum that forms a fibrin clot after centrifugation. Pour off serum as soon as possible, and repeat centrifugation step if more serum is needed.

7.   If there are cells in the poured off specimen, centrifuge the tube and pour off serum into a second Mayo Metal-Free Specimen Vial (Supply T173).

8.   Place the cap on the vial tightly and send the specimen to the laboratory at the temperature indicated in the test’s transport instructions.

Additional Codes

Mayo Test ID
NIS

Reporting Name

Nickel, S

Useful For

Confirmation of an elevated urinary nickel concentration

 

This test is not useful for the investigation of nickel hypersensitivity.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


Urine is the preferred specimen type for determining nickel exposure and potential toxicity. Order NIU / Nickel, 24 Hour, Urine or NIUCR / Nickel/Creatinine Ratio, Random, Urine.

 

This test should not be ordered for the investigation of nickel hypersensitivity. Instead, order testing for lymphocyte proliferation to nickel.



Specimen Required


Patient Preparation: High concentrations of gadolinium and iodine are known to interfere with most metal tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for 96 hours.

Supplies: Metal Free Specimen Vial (T173)

Collection Container/Tube: Royal blue-top (metal-free, no additive)

Submission Container/Tube: 7-mL Mayo metal-free, screw-capped, vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Allow the specimen to clot for 30 minutes; then centrifuge the specimen to separate serum from the cellular fraction.

2. Remove the stopper. Carefully pour specimen into a Mayo metal-free, screw-capped vial, avoiding transfer of the cellular components of blood. Do not insert a pipet into the serum to accomplish transfer, and do not ream the specimen with a wooden stick to assist with serum transfer.

3. See Metals Analysis Specimen Collection and Transport for complete instructions.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days METAL FREE
  Ambient  7 days METAL FREE
  Frozen  7 days METAL FREE

Reference Values

<2.0 ng/mL

Day(s) Performed

Thursday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83885

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NIS Nickel, S 5702-6

 

Result ID Test Result Name Result LOINC Value
8622 Nickel, S 5702-6

Report Available

2 to 8 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)