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Test Code LAB000 - Enter Specimen type, Source, and Test Name T-Cell Receptor Gene Rearrangement, PCR, Varies

Additional Codes

Mayo Test ID
TCGRV

Reporting Name

T Cell Receptor Gene Rearrange, V

Useful For

Determining whether a T-cell population is polyclonal or monoclonal using body fluid or tissue specimens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Body fluid or spinal fluid specimens must arrive within 4 days of collection.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Paraffin-embedded tissue

Container/ Tube: Paraffin block

Collection Instructions:

1. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

2. Indicate specimen source.

3. Include pathology report.

Specimen Stability Information: Ambient

Additional Information: If the quality of the biopsy specimen is poor, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.

 

Acceptable:

Specimen Type: Tissue slide

Slides: 20 Unstained slides

Container/Tube: Transport in plastic slide holders

Collection Instructions:

1. Send 20 unstained, nonbaked slides with 5-micron thick sections of tissue.

2. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

3. Indicate specimen source.

4. Include pathology report.

Specimen Stability Information: Ambient

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.

 

Specimen Type: Body fluid

Sources: Pleural, peritoneal, vitreous and spinal fluid

Container/Tube: Sterile container

Specimen Volume: At least 5 mL

Collection Instructions:

1. If the volume is large, pellet cells prior to sending.

2. Send less volume at ambient temperature or as a frozen cell pellet.

3. Specify the type of fluid being submitted.

Specimen Stability Information:

Body fluid: Ambient 4 days/Refrigerated/Frozen

Cell pellet: Frozen

 

Specimen Type: Frozen tissue

Container/Tube: Plastic container

Specimen Volume: 100 mg

Collection Instructions:

1. Freeze tissue within 1 hour of collection.

2. Indicate specimen source.

Specimen Stability Information: Frozen

 

Specimen Type: Extracted DNA

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted within 7 days of collection.

2. Label specimen as extracted DNA and source of specimen.

3. Provide volume and concentration of DNA on label.

Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.


Specimen Minimum Volume

Body fluid: 1 mL; Frozen tissue: 50 mg; Extracted DNA: 50 microliters (mcL) at 20 ng/mcL; Tissue slides: 10 unstained slides

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81340-TCB (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s), using amplification methodology (eg, PCR)

81342-TCG (T cell receptor, gamma) (eg, leukemia and lymphoma), gene rearrangement analysis, evaluation to detect abnormal clonal population(s)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCGRV T Cell Receptor Gene Rearrange, V In Process

 

Result ID Test Result Name Result LOINC Value
19936 Final Diagnosis: 22637-3
MP016 Specimen: 31208-2
608953 Signing Pathologist 19139-5

Report Available

7 to 14 days

Reject Due To

Bone marrow core biopsies Reject
Paraffin shavings Reject

Method Name

Polymerase Chain Reaction (PCR)

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.